Patrycja Wójcik,
PhD, Eng
Research Assistant
Phone: +48 12 6395 218
Room: 239
E-mail: patrycja.wojcik@ikifp.edu.pl
Research Topics:
- Culture of recombinant bacteria and purification of enzymes
- Studies of enzyme substrate spectrum
- Kinetic studies of enzymatic reactions by the stopped flow method
- Investigation of the mechanism using a kinetic isotope effect
- Research on the use of whole bacterial cells in chemical synthesis
- Protein crystallography
Methods:
- Stopped flow method
- Kinetic isotope effect
- Bacterial fermentation
- Protein crystallization
ORCID: 0000-0001-8317-0686
Selected papers:
-
P. Wojcik, M. Glanowski, B. Mrugala, M. Procner, O. Zastawny, M. Flejszar, K. Kurpiewska, E. Niedzialkowska, W. Minor, M. Oszajca, A. J. Bojarski, A. Wojtkiewicz, M. Szaleniec, “Structure, Mutagenesis, and QM:MM Modeling of 3-Ketosteroid Δ1-Dehydrogenase from Sterolibacterium denitrificans ─ The Role of a New Putative Membrane-Associated Domain and Proton-Relay System in Catalysis”, Biochemistry 62 (2023) 808-823
-
M. Glanowski, P. Wójcik, M. Procner, T. Borowski, D. Lupa, P. Mielczarek, M. Oszajca, K. Świderek, V. Moliner, A.J. Bojarski, M. Szaleniec, "Enzymatic Δ1‑Dehydrogenation of 3‑Ketosteroids - Reconciliation of Kinetic Isotope Effects with the Reaction Mechanism", ACS Catal., 11 (2021) 8211–8225
-
P. Wójcik, M. Glanowski, A. M. Wojtkiewicz, A. Rohman, M. Szaleniec, "Universal capability of 3‑ketosteroid Δ1‑dehydrogenases to catalyze Δ1‑dehydrogenation of C17‑substituted steroids", Microb. Cell Fact., 20 (2021) 119
-
A. M. Wojtkiewicz, P. Wójcik, M. Procner, M. Flejszar, M. Oszajca, M. Hochołowski, M. Tataruch, B. Mrugała, T. Janeczko, M. Szaleniec, "The efficient Δ1-dehydrogenation of a wide spectrum of 3-ketosteroids in a broad pH range by 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans", J. Steroid Biochem. Mol. Biol., 202 (2020) 105731
-
K. Sofińska, A. M. Wojtkiewicz, P. Wójcik, O. Zastawny, M. Guzik, A. Winiarska, P. Waligórski, M. Cieśla, J. Barbasz, M. Szaleniec, "Investigation of quaternary structure of aggregating 3-ketosteroid dehydrogenase from Sterolibacterium denitrificans: In the pursuit of consensus of various biophysical techniques", Biochim. Biophys. Acta-Gen. Subj., 1863 (2019) 1027-1039