Biochemical equipment

Microbiology:

  • Biostat A fermentation station (2x5L) (Sartorious stedim)

  • 2x5L fermentor (Control flow)

  • Incubator with a temperature regulator and shaker (Binder)

  • MaxQ ™ 6000 Thermo Scientific ™ shaker incubator (2x)

  • Shaking incubator New Brunswick Excella E24R

  • Two incubated shakers LabCampanion (ISS-4075R)

  • Autoclave Tuttnauer D-line 5075 ELV

  • Sonics Vibra-Cell VCX500 ultrasonic disintegrator

  • High-pressure laboratory homogenizer EmulsiFlex-C3 (Avestin)

  • Preparative centrifuge Hettich RotoSilenta 630 RS - 6 x 1L

 

Protein purification:

  • Cold room laboratory for biochemical investigations 

  • Anaerobic glove-box for protein purification

  • Liquid chromatograph FPLC AKTApurifier UPC 10 with UPC-900 UV detector and fraction collector (in anaerobic glove-box)

  • Liquid preparative chromatograph FPLC NGC Quest 10 Plus system (Biorad)

  • Liquid chromatograph GE AKTA Start

  • Ultracentrifuges with a set of rotors (Beckman)

  • High-speed centrifuge Hermle Z36HK

  • Vacuum sample concentrator (Labconco-USA)

  • Freeze dryer (Labconco-USA)

  • Deep freezers of IPP PAS (-70°C)

  • Ultra-low Freezer PLATINUM Next V -86°C

 

Analytical:

  • HPLC with detection:

    • UV-Vis (200-800 nm),

    • fluorescence (200-800 nm)

    • electrochemical (COULOCHEM II).

  • Agilent 1100 VL LC-MS with:

    • DAD detector,

    • Electrospray Ionization,

    • Atmospheric Pressure Chemical Ionization.

  • Chromatograph with UV-VIS detector (Shimadzu) for GPC

  • Agilent 1290 Infinity UHPLC with 6460 Triple Quad MS/MS detector​ (QQQ)

    • DAD detector,

    • Electrospray Ionization,

    • Atmospheric Pressure Chemical Ionization.

  • Capillary electrophoresis apparatus with DAD detection (Hewlett Packard)

  • Gas chromatograph Varian CP-3800 with TCD and FID 

  • Bio-Rad gel electrophoresis system for proteins and DNA

  • SUBMINI for fast DNA electrophoresis

  • Baker Solid Phase Extraction chamber

  • CFX Connect Real-Time PCR Detection System

  • Microscope Olympus SZX10

Biophysical equipment

  • UV/Vis spectrophotometers with thermostatic measuring chambers:

    • Spectrophotometer UV/vis Shimadzu UV-1280 with Peltier thermostatic in the anaerobic chamber

    • Spectrophotometer UV/vis Shimadzu UV-2700 with Peltier thermostatic and RX 2000 stopped-flow module (Photophysics)

    • Epoch Microplate Spectrophotometer (BioTek Instruments)

  • CFX Connect Real-Time PCR Detection System

  • Isothermal Titration Calorimeter - Thermal Activity Monitor (TAM III, Thermometric) with:

    • 4 ml Ampule Micro Calorimetric Unit,

    • 4 ml Combined Twin Ampule/Flow/Mix Micro Calorimetric Unit,

    • 4 ml Perfusion/Titration Ampule,

 

Computer modeling facilities

  • Access to CYFRONET supercomputer center - PL-GRID supercomputers - calculation in Gaussian 2016, Turbomole, Amber, etc. 

  • Two computer clusters consisting of servers with multiple-core AMD Opteron processors (+ NVIDIA CUDA GPU)

    • ​Gaussian 09,

    • Jaguar,

    • Molcas Programs for classical simulations: Amber, NAMD2, AutoDock, Modeller

  • Dynochem software package for bioprocess modeling and scaling (Scale-Up Systems)​

What do we do

Our aim is to combine the research potentials of Institute of Catalysis and Surface Chemistry and Institute of Plant Physiology in the field of biochemistry and biotechnology

Our Projects

Laboratory members aplly for new domestic and international projects in order to carry out increasingly advanced research

Join our team

We are open to scientists willing to cooperate on ongoing projects as well as those coming with their own scientific ideas

Contact us

If you are interested in research conducted in our laboratory or have any questions, please do not hesitate and feel free to contact us