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  • Writer's pictureMaciej Szaleniec

Crystal structure of 3-ketosteroid Δ1-dehydrogenase from S. denitrificans in PDB database

Crystal structure of 3-ketosteroid delta1-dehydrogenase from Sterolibacterium denitrificans in complex with 1,4-androstadiene-3,17-dione has been finally released into PDB database. The PDB code of the enzyme is 7P18. The enzyme was crystallized by Patrycja Wójcik, solved by Beata Mrugała and refined by Katarzyna Kurpiewska, Beata and Patrycja. The resolution of the obtained structure was 1.84 Å. The enzyme is the second know structure of 3-ketosteroid dehydrogenase that introduced a double bond between C1 and C2 atoms of the steroid ring and the first example of the enzyme with an additional sequence "loop" at the entrance to the active site. The "loop" turned out to be two helices joined by turn. This discovery is a result of NCN project “The mechanism of regioselective oxidative dehydrogenation of3-ketosteroids catalyzed by Δ1-cholest-4-en-3-one dehydrogenase from Sterolibacterium denitrificans

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