Foto: Maciej Szaleniec
  • Investigation of enzymatic substrates spectrum and analysis of reaction products with LC-MS and GC-MS methods.

  • The study of enzyme reaction mechanisms with steady-state and pre-steady state kinetics 

  • Investigation of enzyme's thermodynamics using isothermal titration calorimetry (ITC).

  • Enzyme inhibition and deactivation study

  • Reaction engineering of enzymatic systems

  • Quantum chemical modeling of reaction pathways of enzymes

  • Modeling of quantitative structure-activity relationships (QSAR) of catalytic activity with Multiple Linear Regression (MLR) and Artificial Neural Network (ANN) methods.

  • Enzyme production in recombinant bacterial systems

  • FPLC and electrophoresis techniques for enzyme purification.

  • Investigation of enzyme activities towards different substrates with spectroscopic and HPLC-based activity assays. Rapid mixing stopped-flow kinetic measurements of half-cycle redox reactions.

  • Experiments with isotope-labeled substrates for determination of isotope kinetic effect. 

  • LC-MS and GC-MS for separation of reaction's products.

  • Isothermal titration calorimetry for investigation of thermodynamics of enzyme reaction.

  • Molecular dynamic (MD) simulations of enzyme-substrate complexes

  • Quantum chemical modeling of cluster model of enzyme active sites and verification of hypothetical reaction mechanism with various substrates.

  • QM:MM modeling of enzyme reactions

  • Prediction of biological activity with ANN and QSAR equations based on quantum chemical parameters.

Prof. Maciej Szaleniec

Head of JLBEC

Member of the ICSC since: October, 2002
Phone: +48 12 6395 101,
Room: 203, 223, 226
E-mail:

ncszalen@cyf-kr.edu.pl
maciej.szaleniec@ikifp.edu.pl

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