Prof. Maciej Szaleniec

Investigation of enzymatic substrates spectrum and analysis of reaction products with LC-MS and GC-MS methods.
The study of enzyme reaction mechanisms with steady-state and pre-steady state kinetics
Investigation of enzyme's thermodynamics using isothermal titration calorimetry (ITC).
Enzyme inhibition and deactivation study
Reaction engineering of enzymatic systems
Quantum chemical modeling of reaction pathways of enzymes
Modeling of quantitative structure-activity relationships (QSAR) of catalytic activity with Multiple Linear Regression (MLR) and Artificial Neural Network (ANN) methods.

Enzyme production in recombinant bacterial systems
FPLC and electrophoresis techniques for enzyme purification.
Investigation of enzyme activities towards different substrates with spectroscopic and HPLC-based activity assays. Rapid mixing stopped-flow kinetic measurements of half-cycle redox reactions.
Experiments with isotope-labeled substrates for determination of isotope kinetic effect.
LC-MS and GC-MS for separation of reaction's products.
Isothermal titration calorimetry for investigation of thermodynamics of enzyme reaction.
Molecular dynamic (MD) simulations of enzyme-substrate complexes
Quantum chemical modeling of cluster model of enzyme active sites and verification of hypothetical reaction mechanism with various substrates.
QM:MM modeling of enzyme reactions
Prediction of biological activity with ANN and QSAR equations based on quantum chemical parameters.

Head of JLBEC

Member of the ICSC since: October, 2002
Phone: +48 12 6395 101,
Room: 203, 223, 226
E-mail: