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Investigation of enzymatic substrates spectrum and analysis of reaction products with LC-MS and GC-MS methods.
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The study of enzyme reaction mechanisms with steady-state and pre-steady state kinetics
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Investigation of enzyme's thermodynamics using isothermal titration calorimetry (ITC).
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Enzyme inhibition and deactivation study
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Reaction engineering of enzymatic systems
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Quantum chemical modeling of reaction pathways of enzymes
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Modeling of quantitative structure-activity relationships (QSAR) of catalytic activity with Multiple Linear Regression (MLR) and Artificial Neural Network (ANN) methods.
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Enzyme production in recombinant bacterial systems
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FPLC and electrophoresis techniques for enzyme purification.
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Investigation of enzyme activities towards different substrates with spectroscopic and HPLC-based activity assays. Rapid mixing stopped-flow kinetic measurements of half-cycle redox reactions.
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Experiments with isotope-labeled substrates for determination of isotope kinetic effect.
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LC-MS and GC-MS for separation of reaction's products.
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Isothermal titration calorimetry for investigation of thermodynamics of enzyme reaction.
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Molecular dynamic (MD) simulations of enzyme-substrate complexes
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Quantum chemical modeling of cluster model of enzyme active sites and verification of hypothetical reaction mechanism with various substrates.
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QM:MM modeling of enzyme reactions
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Prediction of biological activity with ANN and QSAR equations based on quantum chemical parameters.
Prof. Maciej Szaleniec
Head of JLBEC
Member of the ICSC since: October, 2002
Phone: +48 12 6395 101,
Room: 203, 223, 226
E-mail: