• Investigation of enzymatic substrates spectrum and analysis of reaction products with LC-MS and GC-MS methods.

  • The study of enzyme reaction mechanisms with steady-state and pre-steady state kinetics 

  • Investigation of enzyme's thermodynamics using isothermal titration calorimetry (ITC).

  • Enzyme inhibition and deactivation study

  • Reaction engineering of enzymatic systems

  • Quantum chemical modeling of reaction pathways of enzymes

  • Modeling of quantitative structure-activity relationships (QSAR) of catalytic activity with Multiple Linear Regression (MLR) and Artificial Neural Network (ANN) methods.

  • Enzyme production in recombinant bacterial systems

  • FPLC and electrophoresis techniques for enzyme purification.

  • Investigation of enzyme activities towards different substrates with spectroscopic and HPLC-based activity assays. Rapid mixing stopped-flow kinetic measurements of half-cycle redox reactions.

  • Experiments with isotope-labeled substrates for determination of isotope kinetic effect. 

  • LC-MS and GC-MS for separation of reaction's products.

  • Isothermal titration calorimetry for investigation of thermodynamics of enzyme reaction.

  • Molecular dynamic (MD) simulations of enzyme-substrate complexes

  • Quantum chemical modeling of cluster model of enzyme active sites and verification of hypothetical reaction mechanism with various substrates.

  • QM:MM modeling of enzyme reactions

  • Prediction of biological activity with ANN and QSAR equations based on quantum chemical parameters.

Prof. Maciej Szaleniec

Head of JLBEC

Member of the ICSC since: October, 2002
Phone: +48 12 6395 101,
Room: 203, 223, 226
E-mail:

ncszalen@cyf-kr.edu.pl
maciej.szaleniec@ikifp.edu.pl

Methods:

Research Topics:

What do we do

Our aim is to combine the research potentials of Institute of Catalysis and Surface Chemistry and Institute of Plant Physiology in the field of biochemistry and biotechnology

Our Projects

Laboratory members aplly for new domestic and international projects in order to carry out increasingly advanced research

Join our team

We are open to scientists willing to cooperate on ongoing projects as well as those coming with their own scientific ideas

Contact us

If you are interested in research conducted in our laboratory or have any questions, please do not hesitate and feel free to contact us